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FOXO4 Rekombinanter Antikörper

FOXO4 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250921F4

Kat-Nr. : 86236-1-PBS

Synonyme

AFX, AFX1, MLLT7

Filter:
  • Western Blot (WB) analysis of various lysates using FOXO4 Recombinant antibody (86236-1-RR)

    WB analysis using 86236-1-RR (same clone as 86236-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86236-1-RR (FOXO4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86236-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using FOXO4 Recombinant antibody (86236-1-RR)

    IF Staining of HEK-293T using 86236-1-RR (same clone as 86236-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using FOXO4 antibody (86236-1-RR, Clone: 250921F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86236-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86236-1-RR

    Affinity and Kinetic Characterization of 86236-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86236-1-RR against Human FOXO4 were performed. The affinity constant is 2.40 nM.

Geprüfte Anwendungen

Produktinformation

86236-1-PBS bindet in WB, IF/ICC, Indirect ELISA FOXO4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen FOXO4 fusion protein Ag16169
Vollständiger Name forkhead box O4
Berechnetes Molekulargewicht 505 aa, 54 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC106761
Gene symbol FOXO4
Gene ID (NCBI) 4303
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias.