FOXO4 Rekombinanter Antikörper

FOXO4 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250921F4

Kat-Nr. : 86236-1-RR

Synonyme

AFX, AFX1, MLLT7

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  WB analysis using 86236-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86236-1-RR (FOXO4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293T using 86236-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using FOXO4 antibody (86236-1-RR, Clone: 250921F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86236-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86236-1-RR against Human FOXO4 were performed. The affinity constant is 2.40 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293T-Zellen, Jurkat-Zellen, Mausherzgewebe, PC-12-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86236-1-RR bindet in WB, IF/ICC, ELISA FOXO4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXO4 fusion protein Ag16169
• Vollständiger Name: forkhead box O4
• Berechnetes Molekulargewicht: 505 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC106761
• Gene symbol: FOXO4
• Gene ID (NCBI): 4303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias.