FOXO6 Polyklonaler Antikörper

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• KD/KO Validated

FOXO6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19122-1-PBS

Synonyme

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  WB analysis of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19122-1-AP (same clone as 19122-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

  IP result of anti-FOXO6 (IP:19122-1-AP, 3ug; Detection:19122-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 19122-1-AP (same clone as 19122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 19122-1-AP (same clone as 19122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 19122-1-AP (same clone as 19122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 19122-1-AP (same clone as 19122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 19122-1-AP (same clone as 19122-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 19122-1-AP (same clone as 19122-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19122-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA FOXO6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: forkhead box protein O6
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: AI361654
• Gene symbol: FOXO6
• Gene ID (NCBI): 100132074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has an isoform of 70 kDa when modified after translation (PMID:23639108).