FOXP1 Polyklonaler Antikörper

FOXP1 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 22051-1-AP

Synonyme

Mac-1-regulated forkhead, HSPC215, hFKH1B, Forkhead box protein P1, forkhead box P1

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  WB analysis of Jurkat using 22051-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22051-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22051-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22051-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 22051-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 22051-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 22051-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 22051-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 22051-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22051-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22051-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22051-1-AP (FOXP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse embryo using 22051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 22051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 22051-1-AP (FOXP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 22051-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FOXP1 (22051-1-AP) and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, LNCaP-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen, Mausherzgewebe, Mauslungengewebe, Maushodengewebe, PC-3-Zellen, Raji-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

22051-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA FOXP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXP1 fusion protein Ag17045
• Vollständiger Name: forkhead box P1
• Berechnetes Molekulargewicht: 677 aa, 75 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 60-65 kDa, 85 kDa
• GenBank-Zugangsnummer: BC131720
• Gene symbol: FOXP1
• Gene ID (NCBI): 27086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXP1, also known as Mac-1-regulated forkhead, is a 677 amino acid protein, which forms homodimers and heterodimers with FOXP2 and FOXP4 (PubMed:25027557). Dimerization is required for DNA-binding. FOXP1 has an important function in neuronal development.9 Mutations of its gene, FOXP1, located on chromosome 3p14.1,7 can result in the development of autism spectrum disorder, intellectual disability, speech and language deficits as well as motor development delay. FOXP1 is also engaged in lung and esophagus morphogenesis, as well as in B-cell development.7,10 The widely researched role of FOXP1 in carcinogenesis is of great importance, although still unclear to some extent. FOXP1 exists some isoforms with MV 75-77 kDa, 65-67 kDa, 12 kDa.

Publikationen

Species	Application	Title
human,mouse	WB	Int Immunopharmacol; Long non-coding RNA NEAT1 exacerbates NLRP3-mediated pyroptosis in allergic rhinitis through regulating the PTBP1/FOXP1 cascade; Authors - Yunliang Liu
human	WB	J Cell Mol Med; LINC01116 promotes proliferation and migration of endometrial stromal cells by targeting FOXP1 via sponging miR-9-5p in endometriosis.; Authors - Liangyi Cui
human,mouse	WB,IF	J Neurochem; FOXP1 is a Transcription Factor for the Alzheimer's Disease Risk Gene SORL1; Authors - Xiaofeng Fu ; KD Validated