FOXP3 Polyklonaler Antikörper

FOXP3 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

56

Rezensionen (1)

Kat-Nr. : 22228-1-AP

Synonyme

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

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Galerie der Validierungsdaten

• 

  WB analysis of multi-cells/tissue using 22228-1-AP

  WB results of 22228-1-AP (FOXP3 antibody).

• 

  WB analysis of HeLa using 22228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22228-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 22228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22228-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 22228-1-AP

  WB results of anti-22228-1-AP (FOXP3 antibody).

• 

  IP experiment of HeLa using 22228-1-AP

  IP Result of anti-FOXP3 (IP:22228-1-AP, 3ug; Detection:22228-1-AP 1:1000) with HeLa cells lysate 1840ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, HEK-293-Zellen, Maushirngewebe, Multizellen, Multizellen/-gewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 35 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

22228-1-AP bindet in WB, IP, FC, CoIP, ChIP, ELISA FOXP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FOXP3 fusion protein Ag17571
• Vollständiger Name: forkhead box P3
• Berechnetes Molekulargewicht: 431 aa, 47 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC113401
• Gene symbol: FOXP3
• Gene ID (NCBI): 50943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publikationen

Species	Application	Title
mouse		Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
mouse		Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
mouse		Biomaterials; LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.; Authors - Feng Liu
human	WB	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
human	WB	J Autoimmun; NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.; Authors - Mengxiao Xie
rat	WB	Biomed Pharmacother; Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.; Authors - Yu Long

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: brain slices from mouse

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