FOXP3 Polyklonaler Antikörper
FOXP3 Polyklonal Antikörper für WB, IHC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IHC, IF-P, IP, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 22228-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, A375-Zellen |
| Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | humanes Tonsillitisgewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 57 publications below |
| IHC | See 27 publications below |
| IF | See 16 publications below |
| IP | See 2 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 2 publications below |
Produktinformation
22228-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA FOXP3 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | FOXP3 fusion protein Ag17571 |
| Vollständiger Name | forkhead box P3 |
| Berechnetes Molekulargewicht | 431 aa, 47 kDa |
| Beobachtetes Molekulargewicht | 50-55 kDa |
| GenBank-Zugangsnummer | BC113401 |
| Gene symbol | FOXP3 |
| Gene ID (NCBI) | 50943 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for FOXP3 antibody 22228-1-AP | Protokoll herunterladen |
| IHC protocol for FOXP3 antibody 22228-1-AP | Protokoll herunterladenl |
| IF protocol for FOXP3 antibody 22228-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy | ||
Cancer Res Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma. | ||
Biomaterials LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy. | ||
Clin Transl Med MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis. | ||
Biomed Pharmacother Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Anne-Laure (Verified Customer) (12-07-2021) | non-specific staining
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