FOXP4 Rekombinanter Antikörper

FOXP4 Rekombinant Antikörper für IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242356B4

Kat-Nr. : 84855-1-PBS

Synonyme

242356B4, FKHLA, Fork head-related protein-like A

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  Sandwich ELISA standard curve of MP01621-2

  Sandwich ELISA standard curve of MP01621-2, Human FOXP4 Recombinant Matched Antibody Pair - PBS only. 84855-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10047. 84855-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01621-2

  The mean FOXP4 concentration was determined to be 5.4 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

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  IF Staining of HepG2 using 84855-1-RR (same clone as 84855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84855-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84855-1-RR (same clone as 84855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84855-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84855-1-RR (same clone as 84855-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXP4 Recombinant antibody (84855-1-RR, Clone:242356B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84855-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84855-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84855-1-RR against Human FOXP4 were performed. The affinity constant is 0.402 nM.

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Geprüfte Anwendungen

Produktinformation

84855-1-PBS bindet in IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test FOXP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXP4 fusion protein Ag10047
• Vollständiger Name: forkhead box P4
• Berechnetes Molekulargewicht: 678 aa, 73 kDa
• GenBank-Zugangsnummer: BC052803
• Gene symbol: FOXP4
• Gene ID (NCBI): 116113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.