FOXP4 Rekombinanter Antikörper

FOXP4 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242356B4

Kat-Nr. : 84855-1-RR

Synonyme

Fork head-related protein-like A, FKHLA, 242356B4

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  IF Staining of HeLa using 84855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84855-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody (84855-1-RR, Clone: 242356B4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 84855-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXP4 Recombinant antibody (84855-1-RR, Clone:242356B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84855-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84855-1-RR against Human FOXP4 were performed. The affinity constant is 0.402 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84855-1-RR bindet in IF/ICC, FC (Intra), ELISA FOXP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FOXP4 fusion protein Ag10047
• Vollständiger Name: forkhead box P4
• Berechnetes Molekulargewicht: 678 aa, 73 kDa
• GenBank-Zugangsnummer: BC052803
• Gene symbol: FOXP4
• Gene ID (NCBI): 116113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.