FPGS Polyklonaler Antikörper

FPGS Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27616-1-PBS

Synonyme

Tetrahydrofolate synthase

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  WB analysis using 27616-1-AP (same clone as 27616-1-PBS)

  HeLa mitochondrial extracts were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 27616-1-AP (same clone as 27616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 27616-1-AP (same clone as 27616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27616-1-AP (same clone as 27616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 27616-1-AP (same clone as 27616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27616-1-PBS bindet in WB, IHC, Indirect ELISA FPGS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FPGS fusion protein Ag24775
• Vollständiger Name: folylpolyglutamate synthase
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC064393
• Gene symbol: FPGS
• Gene ID (NCBI): 2356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.