FRY Polyklonaler Antikörper

FRY Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32075-1-PBS

Synonyme

C13orf14, FRY Microtubule Binding Protein, Protein furry homolog

---

• 

  WB analysis using 32075-1-AP (same clone as 32075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32075-1-AP (FRY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 32075-1-AP (same clone as 32075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 32075-1-AP (same clone as 32075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 32075-1-AP (same clone as 32075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 32075-1-AP (same clone as 32075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FRY antibody (32075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

32075-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FRY und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FRY fusion protein Ag34959
• Vollständiger Name: furry homolog (Drosophila)
• Berechnetes Molekulargewicht: 339 kDa
• Beobachtetes Molekulargewicht: 340 kDa
• GenBank-Zugangsnummer: NM_023037
• Gene symbol: FRY
• Gene ID (NCBI): 10129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The FRY gene encodes a protein involved in cell cycle regulation and DNA damage response. It plays a crucial role in the G2/M checkpoint, ensuring cells complete DNA repair before entering mitosis by activating the ATR-CHK1 signaling pathway. FRY is expressed in various tissues, including brain, liver, and testis, with notable expression in Sertoli and spermatogonial cells. Aberrant FRY function is associated with multiple diseases, such as cancer and neurodegenerative disorders. In cancer cells, FRY expression can influence proliferation and survival. Understanding FRY's role in cell cycle control and genome stability is vital for elucidating disease mechanisms and developing targeted therapies.