FRZB Monoklonaler Antikörper

FRZB Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2B4A1

Kat-Nr. : 67081-1-Ig

Synonyme

FIZ, FRE, Frezzled, FRITZ, frizzled related protein, Frizzled related protein 1, FRP, FRP 3, FRZB, FRZB 1, FRZB PEN, FRZB1, FZRB, hFIZ, sFRP 3, SFRP3, SRFP3

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  WB analysis using 67081-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67081-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, MKN-45-Zellen, SGC-7901-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67081-1-Ig bindet in WB, ELISA FRZB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FRZB fusion protein Ag28147
• Vollständiger Name: frizzled-related protein
• Berechnetes Molekulargewicht: 325 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC027855
• Gene symbol: FRZB
• Gene ID (NCBI): 2487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.

Publikationen

Species	Application	Title
human	WB	Eur J Pharmacol; Eltrombopag restores proliferative capacity and adipose-osteogenic balance of mesenchymal stromal cells in low-risk myelodysplastic syndromes; Authors - Minghua Hong
human	WB	Stem Cells Dev; Chlorogenic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Wnt Signaling.; Authors - Xiaoping Hu