Follistatin Monoklonaler Antikörper

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• KD/KO Validated

Follistatin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

4E9B10

Rezensionen (1)

Kat-Nr. : 60060-1-Ig

Synonyme

FST, FS, Activin-binding protein, Activin binding protein, 4E9B10

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  WB analysis using 60060-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60060-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60060-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 60060-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 60060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60060-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Follistatin antibody (60060-1-Ig, Clone: 4E9B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, NCI-H1299-Zellen, Raji-Zellen, SKOV-3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

60060-1-Ig bindet in WB, IHC, IF/ICC, ELISA Follistatin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Follistatin fusion protein Ag0775
• Vollständiger Name: follistatin
• Berechnetes Molekulargewicht: 33 aa, 7 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC004107
• Gene symbol: Follistatin
• Gene ID (NCBI): 10468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome. FS 288 glycosylation results in a major species of 35 kDa and a less abundant 40 kDa and minor products of 40 and 42 kDa (PMID: 9785474). Three typical follistatin bands are at 32, 35, and 39 kDa (PMID: 2036994).

Publikationen

Species	Application	Title
rat	IHC	Cells; Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage; Authors - Izumi Nagayama
mouse	IHC	Antioxid Redox Signal; Follistatin Protects against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease.; Authors - Neel Mehta
mouse	WB	Front Pharmacol; Follistatin Attenuates Myocardial Fibrosis in Diabetic Cardiomyopathy via the TGF-β-Smad3 Pathway.; Authors - Yinhui Wang
human	IF	Fertil Steril; Myostatin, follistatin and activin type II receptors are highly expressed in adenomyosis.; Authors - Patrizia Carrarelli
human,mouse	WB,IF	PeerJ; Development of a gene doping detection method to detect overexpressed human follistatin using an adenovirus vector in mice.; Authors - Koki Yanazawa
camel	WB,IF	Animals (Basel); Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels.; Authors - Qi Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jennifer (Verified Customer) (07-17-2023)

I am not sure what this antibody targets, but I am sure it is not Follistatin. This is a glycosylated protein that appears as a smear at ~40 - 50 kDa. However, with this antibody there is a single band at ~ 35 kDa. We have validated several FST antibodies using knock-down and overexpression cell lines so we know what to expect.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: HNSCC cell lines