FTCD Polyklonaler Antikörper

FTCD Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 21959-1-AP

Synonyme

Glutamate formyltransferase, Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase

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  WB analysis using 21959-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21959-1-AP (FTCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21959-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21959-1-AP (FTCD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21959-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21959-1-AP (FTCD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:4000-1:16000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

21959-1-AP bindet in WB, IHC, IF, ELISA FTCD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FTCD fusion protein Ag16646
• Vollständiger Name: formiminotransferase cyclodeaminase
• Berechnetes Molekulargewicht: 541 aa, 59 kDa
• Beobachtetes Molekulargewicht: 53-62 kDa
• GenBank-Zugangsnummer: BC136395
• Gene symbol: FTCD
• Gene ID (NCBI): 10841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

Publikationen

Species	Application	Title
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
human	IF	Front Cell Dev Biol; Significance of Liver Zonation in Hepatocellular Carcinoma.; Authors - Shizhe Yu
human	IHC	Bioengineered; A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.; Authors - Huimin Shen
human	WB,IHC	Med Sci Monit; Formiminotransferase Cyclodeaminase Suppresses Hepatocellular Carcinoma by Modulating Cell Apoptosis, DNA Damage, and Phosphatidylinositol 3-Kinases (PI3K)/Akt Signaling Pathway.; Authors - Jiajia Chen
mouse	WB	FASEB J; Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment; Authors - Kenneth N Maclean
Human		iScience; Crosstalk of non-apoptotic RCD panel in hepatocellular carcinoma reveals the prognostic and therapeutic optimization; Authors - Shuo Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (05-11-2022)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: House liver/HepG2

FTCD Antibody Western Blot validation (1:4000 dilution) in House liver/HepG2 (Cat no:21959-1-AP)