FTCD Rekombinanter Antikörper

FTCD Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240654G9

Kat-Nr. : 83664-2-PBS

Synonyme

240654G9, Formimidoyltetrahydrofolate cyclodeaminase, Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, Glutamate formimidoyltransferase

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  WB analysis using 83664-2-RR (same clone as 83664-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83664-2-RR (FTCD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83664-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00636-3

  The mean FTCD concentration was determined to be 114.0 pg/mL in HepG2 cell extract based on a 3.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00636-3

  Sandwich ELISA standard curve of MP00636-3, Human FTCD Recombinant Matched Antibody Pair - PBS only. 83664-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16646. 83664-4-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL.

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  FC experiment of HepG2 using 83664-2-RR (same clone as 83664-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FTCD Recombinant antibody (83664-2-RR, Clone:240654G9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83664-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83664-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83664-2-RR against Human FTCD were performed. The affinity constant is 6.37 nM.

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Geprüfte Anwendungen

Produktinformation

83664-2-PBS bindet in WB, Sandwich ELISA, Indirect ELISA FTCD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FTCD fusion protein Ag16646
• Vollständiger Name: formiminotransferase cyclodeaminase
• Berechnetes Molekulargewicht: 541 aa, 59 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC136395
• Gene symbol: FTCD
• Gene ID (NCBI): 10841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).