Ferritin heavy chain Monoklonaler Antikörper

Ferritin heavy chain Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Kaninchen, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G8D7

Kat-Nr. : 60875-5-Ig

Synonyme

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

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  WB analysis using 60875-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60875-5-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 60875-5-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 60875-5-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 60875-5-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HepG2 using 60875-5-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, K-562-Zellen, Kaninchenhirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60875-5-Ig bindet in WB, IHC, IF/ICC, ELISA Ferritin heavy chain und zeigt Reaktivität mit human, Kaninchen, Ratten

• Getestete Reaktivität: human, Kaninchen, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ferritin heavy chain fusion protein Ag21386
• Vollständiger Name: ferritin, heavy polypeptide 1
• Berechnetes Molekulargewicht: 183 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000857
• Gene symbol: FTH1
• Gene ID (NCBI): 2495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.