Ferritin heavy chain Monoklonaler Antikörper

Ferritin heavy chain Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Kaninchen, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G8D7

Kat-Nr. : 60875-5-PBS

Synonyme

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

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  WB analysis using 60875-5-Ig (same clone as 60875-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

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  WB analysis using 60875-5-Ig (same clone as 60875-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60875-5-Ig (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 60875-5-Ig (same clone as 60875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 60875-5-Ig (same clone as 60875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60875-5-Ig (FTH1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60875-5-Ig (same clone as 60875-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60875-5-Ig (same clone as 60875-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ferritin heavy chain antibody (60875-5-Ig, Clone: 3G8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60875-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60875-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Ferritin heavy chain und zeigt Reaktivität mit human, Kaninchen, Ratten

• Getestete Reaktivität: human, Kaninchen, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ferritin heavy chain fusion protein Ag21386
• Vollständiger Name: ferritin, heavy polypeptide 1
• Berechnetes Molekulargewicht: 183 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000857
• Gene symbol: FTH1
• Gene ID (NCBI): 2495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.