Ferritin heavy chain Rekombinanter Antikörper

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Ferritin heavy chain Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230312F3

Kat-Nr. : 83428-1-PBS

Synonyme

Ferritin, FTH1, 230312F3, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1

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  WB analysis of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

  WB result of Ferritin heavy chain antibody (83428-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

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  WB analysis using 83428-1-RR (same clone as 83428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83428-1-RR (same clone as 83428-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (83428-1-RR, Clone: 230312F3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83428-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83428-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83428-1-PBS bindet in WB, IF/ICC, ELISA Ferritin heavy chain und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ferritin heavy chain fusion protein Ag2290
• Vollständiger Name: ferritin, heavy polypeptide 1
• Berechnetes Molekulargewicht: 183 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000857
• Gene symbol: FTH1
• Gene ID (NCBI): 2495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.