Ferritin light chain Monoklonaler Antikörper

Ferritin light chain Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

CloneNo.

1F9F5

Kat-Nr. : 68068-1-Ig

Synonyme

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

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  WB analysis of A549 using 68068-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68068-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68068-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 68068-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68068-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 68068-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 1 publications below

Produktinformation

68068-1-Ig bindet in WB, IHC, IF/ICC, ELISA Ferritin light chain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ferritin light chain fusion protein Ag20637
• Vollständiger Name: ferritin, light polypeptide
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC008439
• Gene symbol: Ferritin light chain
• Gene ID (NCBI): 2512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.

Publikationen

Species	Application	Title
mouse	WB	J Gastroenterol; Indoleamine 2,3-dioxygenase 1-mediated iron metabolism in macrophages contributes to lipid deposition in nonalcoholic steatohepatitis; Authors - Chaofeng Wu
mouse	WB	J Neuroimmune Pharmacol; Ghrelin Induces Ferroptosis Resistance and M2 Polarization of Microglia to Alleviate Neuroinflammation and Cognitive Impairment in Alzheimer's Disease; Authors - Yaoxue Guo
	WB	Inflammopharmacology; Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis; Authors - Chenyu Zhao
mouse	WB	J Nutr Biochem; Curcumol ameliorates alcohol and high-fat diet-induced fatty liver disease via modulation of the Ceruloplasmin/iron overload/mtDNA signaling pathway; Authors - Tingting Ding
human	WB	J Ethnopharmacol; Anti-papillary thyroid carcinoma effects of dioscorea bulbifera L. through ferroptosis and the PI3K/AKT pathway based on network pharmacology and experimental validation; Authors - Yuzhi Gao
mouse	WB	Biochim Biophys Acta Mol Basis Dis; FTO alleviated ferroptosis in septic cardiomyopathy via mediating the m6A modification of BACH1; Authors - Hua Zeng