FTL Rekombinanter Antikörper

FTL Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251048G12

Kat-Nr. : 81166-3-RR

Synonyme

Ferritin, Ferritin light chain, Ferritin L subunit

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  WB analysis using 81166-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81166-3-RR (FTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 81166-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using FTL antibody (81166-3-RR, Clone: 251048G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 81166-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FTL antibody (81166-3-RR, Clone: 251048G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81166-3-RR bindet in WB, IF/ICC, ELISA FTL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FTL fusion protein Ag20637
• Vollständiger Name: ferritin, light polypeptide
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC008439
• Gene symbol: Ferritin light chain
• Gene ID (NCBI): 2512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.