FTO Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FTO Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF-P, FC (Intra), IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(210)

Rezensionen (5)

Kat-Nr. : 27226-1-AP

Synonyme

KIAA1752

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• 

  WB analysis using 27226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-231 using 27226-1-AP

  WB result of FTO antibody (27226-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and FTO knockout MDA-MB-231 cells.

• 

  WB analysis using 27226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lymphoma using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lymphoma using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 27226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 27226-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (27226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen, MDA-MB-231-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lymphomgewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 26 publications below
• WB: See 186 publications below
• IHC: See 43 publications below
• IF: See 21 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below
• RIP: See 18 publications below

Produktinformation

27226-1-AP bindet in WB, IHC, IF-P, FC (Intra), IP, CoIP, RIP, ELISA FTO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FTO fusion protein Ag26095
• Vollständiger Name: fat mass and obesity associated
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_001080432
• Gene symbol: FTO
• Gene ID (NCBI): 79068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Publikationen

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human	WB	Nat Genet; N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.; Authors - Yuan Li ; KO Validated
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human,mouse	WB,IP	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
mouse	WB	Adv Sci (Weinh); PXR Activation Relieves Deoxynivalenol-Induced Liver Oxidative Stress Via Malat1 LncRNA m6A Demethylation; Authors - Yue Feng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaochen (Verified Customer) (07-08-2024)

Show good sepecific image in WB without non-specific bend.

• Applications: Western Blot
• Cell Tissue Type: U2OS

FTO Antibody Western Blot validation ( dilution) in U2OS (Cat no:27226-1-AP)

FH

Katherine (Verified Customer) (09-25-2023)

Quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

S (Verified Customer) (03-20-2023)

Very Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

FTO Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:27226-1-AP)

FH

SU (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: H9C2

FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)

FH

P. (Verified Customer) (05-15-2021)

Excellent antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)