FTO Polyklonaler Antikörper

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• KD/KO Validated

FTO Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27226-1-PBS

Synonyme

KIAA1752

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  WB analysis using 27226-1-AP (same clone as 27226-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-231 using 27226-1-AP (same clone as 27226-1-PBS)

  WB result of FTO antibody (27226-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and FTO knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27226-1-AP (same clone as 27226-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lymphoma using 27226-1-AP (same clone as 27226-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lymphoma using 27226-1-AP (same clone as 27226-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 27226-1-AP (same clone as 27226-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 27226-1-AP (same clone as 27226-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (27226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27226-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27226-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA FTO und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FTO fusion protein Ag26095
• Vollständiger Name: fat mass and obesity associated
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_001080432
• Gene symbol: FTO
• Gene ID (NCBI): 79068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.