FTO Monoklonaler Antikörper

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FTO Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IHC, FC (Intra), RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

1E8B1

Kat-Nr. : 68111-1-Ig

Synonyme

1E8B1, KIAA1752

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  WB analysis using 68111-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 68111-1-Ig

  WB result of FTO antibody (68111-1-Ig; 1:3000; room temperature for 1.5 hours) with wild-type and FTO knockout MDA-MB-231 cells.

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  WB analysis of NIH/3T3 using 68111-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 68111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68111-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 68111-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68111-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 68111-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (68111-1-Ig, Clone:1E8B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, HUVEC-Zellen, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

68111-1-Ig bindet in WB, IHC, FC (Intra), RIP, ELISA FTO und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FTO fusion protein Ag26095
• Vollständiger Name: fat mass and obesity associated
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_001080432
• Gene symbol: FTO
• Gene ID (NCBI): 79068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Publikationen

Species	Application	Title
pig	WB	Int J Biol Macromol; METTL3 enhances E. coli F18 resistance by targeting IKBKG/NF-κB signaling via an m6A-YTHDF1-dependent manner in IPEC-J2 cells; Authors - Jian Jin
mouse	WB	Acta Physiol (Oxf); Landscape of transcriptome-wide m6A modification in diabetic liver reveals rewiring of PI3K-Akt signaling after physical exercise; Authors - Cong Chen
mouse,human	WB,RIP,IHC	Noncoding RNA Res; Upregulation of circGDI2 inhibits tumorigenesis by stabilizing the expression of RNA m6A demethylase FTO in oral squamous cell carcinoma; Authors - Yuwei Gu
rat	WB	Acta Biochim Biophys Sin (Shanghai); Endogenous RBM4 prevents Ang II-induced cardiomyocyte hypertrophy via downregulating the expression of PTBP1; Authors - Weihan Sun
human	WB	Apoptosis; NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer; Authors - Yang Wang