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  • KD/KO Validated

FUBP1 Polyklonaler Antikörper

FUBP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 24864-1-AP

Synonyme

FUSE-binding protein 1, Far upstream element-binding protein 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSH-SY5Y-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe
Erfolgreiche IPSH-SY5Y-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

24864-1-AP bindet in WB, IF/ICC, IP, ChIP, ELISA FUBP1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FUBP1 fusion protein Ag13149
Vollständiger Name far upstream element (FUSE) binding protein 1
Berechnetes Molekulargewicht 653 aa, 69 kDa
Beobachtetes Molekulargewicht 69 kDa
GenBank-ZugangsnummerBC017247
Gene symbol FUBP1
Gene ID (NCBI) 8880
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for FUBP1 antibody 24864-1-APProtokoll herunterladen
IF protocol for FUBP1 antibody 24864-1-APProtokoll herunterladen
IP protocol for FUBP1 antibody 24864-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Poly(U) binding splicing factor 60 promotes renal cell carcinoma growth by transcriptionally upregulating telomerase reverse transcriptase.

Authors - Qian Long
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
humanWB

Anticancer Res

Comparative Proteomic Profiling of Cisplatin-resistant Nasopharyngeal Carcinoma Cell Lines: Novel Biomarkers for Improving Chemotherapy of NPC.

Authors - Parisa Prathaphan
humanWB

J Adv Res

LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1

Authors - Min Jiang
humanChIP

MedComm (2020)

Far upstream element-binding protein 1 confers lobaplatin resistance by transcriptionally activating PTGES and facilitating the arachidonic acid metabolic pathway in osteosarcoma

Authors - Qiong Ma
humanWB

J Immunother Cancer

The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development

Authors - Cong Zhang
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Clean nuclear staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa/human
FUBP1 Antibody Immunofluorescence validation (1:1000 dilution) in HeLa/human (Cat no:24864-1-AP)