FUBP1 Polyklonaler Antikörper

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• KD/KO Validated

FUBP1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24864-1-PBS

Synonyme

FUSE-binding protein 1, Far upstream element-binding protein 1

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  WB analysis of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 24864-1-AP (same clone as 24864-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 24864-1-AP (same clone as 24864-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 24864-1-AP (same clone as 24864-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

• 

  IP experiment of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

  IP result of anti-FUBP1 (IP:24864-1-AP, 4ug; Detection:24864-1-AP 1:300) with SH-SY5Y cells lysate 800ug. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24864-1-AP (same clone as 24864-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUBP1 antibody (24864-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24864-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA FUBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUBP1 fusion protein Ag13149
• Vollständiger Name: far upstream element (FUSE) binding protein 1
• Berechnetes Molekulargewicht: 653 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC017247
• Gene symbol: FUBP1
• Gene ID (NCBI): 8880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.