FUCA2 Polyklonaler Antikörper

FUCA2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 15157-1-AP

Synonyme

Alpha L fucosidase 2, FUCA2, fucosidase, alpha L 2, plasma, Plasma alpha L fucosidase, UNQ227/PRO260

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  WB analysis of human heart using 15157-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15157-1-AP

  IP result of anti-FUCA2 (IP:15157-1-AP, 4ug; Detection:15157-1-AP 1:300) with mouse heart tissue lysate 4000 ug.

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  IHC staining of human liver using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Maus-Magengewebe, Ratten-Magengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15157-1-AP bindet in WB, IHC, IP, ELISA FUCA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUCA2 fusion protein Ag7250
• Vollständiger Name: fucosidase, alpha-L- 2, plasma
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC003060
• Gene symbol: FUCA2
• Gene ID (NCBI): 2519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Commun; Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis; Authors - Xiaoqiong Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gretchen (Verified Customer) (02-01-2019)

I did a 1:2000 dilution in 30ug of lysate protein. The bands were sharp and strong and it took 3 seconds to develop. However, I had a lot of unspecific bands and not only at 86, they were some stronger than others.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Melanoma cells