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FUCA2 Polyklonaler Antikörper

FUCA2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30080-1-PBS

Synonyme

Alpha-L-fucosidase 2, Alpha-L-fucoside fucohydrolase 2, EC:3.2.1.51, Plasma alpha-L-fucosidase, PSEC0151

Filter:
  • Western Blot (WB) analysis of various lysates using FUCA2 Polyclonal antibody (30080-1-AP)

    WB analysis using 30080-1-AP (same clone as 30080-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30080-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FUCA2 Polyclonal antibody (30080-1-AP)

    IHC staining of mouse liver using 30080-1-AP (same clone as 30080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FUCA2 Polyclonal antibody (30080-1-AP)

    IF Staining of HepG2 using 30080-1-AP (same clone as 30080-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA2 antibody (30080-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30080-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FUCA2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FUCA2 fusion protein Ag32467
Vollständiger Name fucosidase, alpha-L- 2, plasma
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht 54-60 kDa
GenBank-ZugangsnummerBC003060
Gene symbol FUCA2
Gene ID (NCBI) 2519
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.