FURIN Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FURIN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Rezensionen (4)

Kat-Nr. : 18413-1-AP

Synonyme

PCSK3, Paired basic amino acid residue-cleaving enzyme, PACE, FUR, EC:3.4.21.75

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  WB analysis of HepG2 using 18413-1-AP

  WB result of FURIN antibody (18413-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FURIN transfected HepG2 cells.

• 

  WB analysis of COLO 320 using 18413-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18413-1-AP (FURIN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 18413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18413-1-AP (FURIN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 18413-1-AP

  IP result of anti-FURIN (IP:18413-1-AP, 4ug; Detection:18413-1-AP 1:4000) with HeLa cells lysate 2000 ug.

• 

  IHC staining of human placenta using 18413-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18413-1-AP (FURIN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 18413-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18413-1-AP (FURIN Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 18413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FURIN antibody (18413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, COLO 320-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

18413-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FURIN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FURIN fusion protein Ag13261
• Vollständiger Name: furin (paired basic amino acid cleaving enzyme)
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 90-110 kDa
• GenBank-Zugangsnummer: BC012181
• Gene symbol: FURIN
• Gene ID (NCBI): 5045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530).

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination; Authors - Wenqiang Su ; KD Validated
human	IHC	Cell Rep; Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury; Authors - Yiheng Mao
human	WB	Oncogene; Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines.; Authors - Diana Farhat
human	WB	PLoS Pathog; Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2; Authors - Shi Yu ; KD Validated
human	WB	Oncogenesis; Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.; Authors - Huizhi Wang
human	WB	Br J Cancer; Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.; Authors - Diana Farhat ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (05-31-2024)

WB at 1:1000. Two (80 and 104 kDa) bands detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines

FH

Wojciech (Verified Customer) (05-21-2024)

Used in WB and got 2 bands at 81 and 104 kDa at 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T-ALL cell lines

FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF

FH

Alex (Verified Customer) (10-23-2018)

Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: 293T, HeLa