FUS/TLS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FUS/TLS Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

112

Rezensionen (8)

Kat-Nr. : 11570-1-AP

Synonyme

75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11570-1-AP

  WB result of FUS antibody (11570-1-AP, 1:5000) with si-Control and si-FUS transfected HEK 293 cells.

• 

  WB analysis of HeLa using 11570-1-AP

  HeLa (WT and FUS KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 11570-1-AP incubated at 1:5000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 11570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11570-1-AP

  IP result of anti-FUS/TLS(IP:11570-1-AP, 4ug; Detection:11570-1-AP 1:2000) with K-562 cells lysate 1760 ug.

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 11570-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11570-1-AP (FUS/TLS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11570-1-AP

  HeLa WT cells (yellow outline) and FUS KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11570-1-AP at 1:2000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IF Staining of HeLa using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUS/TLS antibody (11570-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FUS/TLS antibody (11570-1-AP) at dilution of 1:200, CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange), E-cadherin antibody (20874-1-AP, Magenta).

• 

  FC experiment of HeLa using 11570-1-AP

  1X10^6 HeLa cells were stained with 0.2ug FUS/TLS antibody (11570-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Kolongewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 58 publications below
• IHC: See 32 publications below
• IF: See 46 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 4 publications below

Produktinformation

11570-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA FUS/TLS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUS/TLS fusion protein Ag2150
• Vollständiger Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 68-75 kDa
• GenBank-Zugangsnummer: BC026062
• Gene symbol: FUS
• Gene ID (NCBI): 2521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. FUS was detected double bands of 68-74 kDa (PMID:31519807).

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC,IF	Nature; Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.; Authors - Deng Han-Xiang HX
human,mouse	WB	Nat Med; Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.; Authors - Vladislav A Korobeynikov
human	WB,IF	Cell; Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.; Authors - Lin Guo
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.; Authors - Gina Picchiarelli ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xhuljana (Verified Customer) (03-01-2024)

Used in siRNA transfected C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

FUS/TLS Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:11570-1-AP)

FH

Zhongwen (Verified Customer) (09-25-2023)

I can find two bands in the target region. I am not sure which one is the target band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FUS/TLS Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11570-1-AP)

FH

manohar (Verified Customer) (07-10-2023)

Nitrocellulose membrane is used with 5% milk as blocking and antibody diluted in 1% milk and incubated overnight.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Tatyana (Verified Customer) (05-14-2023)

ICC using 5% goat serum/PBST buffer, 2 hours at RT. Good specific nuclear signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

FUS/TLS Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11570-1-AP)

FH

shashirekha (Verified Customer) (12-23-2020)

Used for immunopreciptation at 1:1000 dilution. Works very well

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat cortical neurons

FUS/TLS Antibody Immunoprecipitation validation (1:1000 dilution) in Rat cortical neurons (Cat no:11570-1-AP)

FH

H (Verified Customer) (04-06-2020)

The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

FUS/TLS Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:11570-1-AP)

FH

Karthik (Verified Customer) (04-24-2019)

Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 overnight at 4 degrees
• Cell Tissue Type: motor neurons

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human motor neuron

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