FUS/TLS Monoklonaler Antikörper

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• KD/KO Validated

FUS/TLS Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B4F8

Kat-Nr. : 68262-1-PBS

Synonyme

FUS, 1B4F8, 75 kDa DNA pairing protein, 75 kDa DNA-pairing protein, ALS

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  WB analysis using 68262-1-Ig (same clone as 68262-1-PBS)

  Untreated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68262-1-Ig (same clone as 68262-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 68262-1-Ig (same clone as 68262-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68262-1-Ig (same clone as 68262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68262-1-Ig (same clone as 68262-1-PBS)

  WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUS/TLS transfected HepG2 cells. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68262-1-Ig (same clone as 68262-1-PBS)

  WB result of FUS/TLS antibody (68262-1-Ig; 1:10000; room temperature for 1.5 hours) with negative control and FUS/TLS knockout HeLa cells. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 68262-1-Ig (same clone as 68262-1-PBS)

  IP result of anti-FUS/TLS (IP:68262-1-Ig, 4ug; Detection:68262-1-Ig 1:5000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68262-1-Ig (FUS/TLS antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), Calbindin-D28k antibody (14479-1-AP, green). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 68262-1-Ig (same clone as 68262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat cerebellum tissue using FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3), Calbindin-D28k antibody (14479-1-AP, green). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293T using 68262-1-Ig (same clone as 68262-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human FUS/TLS (68262-1-Ig, Clone:1B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68262-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68262-1-PBS bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA FUS/TLS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FUS/TLS fusion protein Ag2150
• Vollständiger Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 68-75 kDa
• GenBank-Zugangsnummer: BC026062
• Gene symbol: FUS/TLS
• Gene ID (NCBI): 2521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)(PMID: 22640227). Multiple phosphorylation on the N terminus of FUS caused that FUS was detected 68-75 kDa (PMID:24899704).