FUS/TLS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FUS/TLS Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®488 Fluorescent Dye

Kat-Nr. : CL488-11570

Synonyme

FUS, FUS1, ALS6, ALS, 75 kDa DNA-pairing protein

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  IF Staining of HepG2 using CL488-11570

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®488 FUS/TLS antibody (CL488-11570) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-11570

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®488 FUS/TLS antibody (CL488-11570) at dilution of 1:200.

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  IF Staining of HeLa using CL488-11570

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 FUS/TLS antibody (CL488-11570) at dilution of 1:200, CoraLite®594 TDP-43 (C-terminal) antibody (CL594-12892, red).

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  FC experiment of MCF-7 using CL488-11570

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite®488 Anti-Human FUS/TLS (CL488-11570) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11570 bindet in IF/ICC, FC (Intra) FUS/TLS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUS/TLS fusion protein Ag2150
• Vollständiger Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Berechnetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC026062
• Gene symbol: FUS/TLS
• Gene ID (NCBI): 2521
• Konjugation: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.