FUT4 Polyklonaler Antikörper

FUT4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22141-1-AP

Synonyme

Alpha (1,3) fucosyltransferase, ELFT, FCT3A, FUC TIV, Fucosyltransferase 4, Fucosyltransferase IV, FucT IV, FUT4, FUTIV

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 22141-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22141-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22141-1-AP (FUT4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 22141-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22141-1-AP (FUT4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 22141-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FUT4 antibody (22141-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22141-1-AP bindet in WB, IHC, IF, ELISA FUT4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FUT4 fusion protein Ag17565
• Vollständiger Name: fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Berechnetes Molekulargewicht: 530 aa, 59 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 63 kDa
• GenBank-Zugangsnummer: BC136374
• Gene symbol: FUT4
• Gene ID (NCBI): 2526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation. 22141-1-AP detects the band around 45 kDa and glycosylated isoform 63 kDa protein in SDS-PAGE. (PMID: 22287018, 17335083, 11278338)