Frataxin Polyklonaler Antikörper

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• KD/KO Validated

Frataxin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Rezensionen (3)

Kat-Nr. : 14147-1-AP

Synonyme

FXN, CyaY, d FXN, dFXN, EC:1.16.3.1

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• 

  WB analysis using 14147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14147-1-AP (Frataxin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14147-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14147-1-AP (Frataxin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, Jurkat-Zellen, K-562-Zellen, Mauslebergewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14147-1-AP bindet in WB, IHC, IF, CoIP, ELISA Frataxin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Frataxin fusion protein Ag5316
• Vollständiger Name: frataxin
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 14-23 kDa
• GenBank-Zugangsnummer: BC048097
• Gene symbol: FXN
• Gene ID (NCBI): 2395
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Frataxin (FXN), also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574)

Publikationen

Species	Application	Title
human	WB	Blood; Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.; Authors - Floriane Petit
human	WB	Brain; Autosomal recessive cerebellar ataxia caused by a homozygous mutation in PMPCA.; Authors - Karine Choquet
mouse	WB	Neurobiol Dis; Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin; Authors - Daniel Fil
mouse	WB	J Nutr Biochem; Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction; Authors - Jingjing Liu
human	WB,IHC	Sci Rep; Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich's ataxia.; Authors - Joseph F Nabhan
human	WB	Hum Mol Genet; Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia.; Authors - Yanjie Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mariana (Verified Customer) (01-06-2021)

Failed to detect endogenous FXN in IPSC derived Cardiomyocytes from Friedreich Ataxia patients and controls.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: IPSC cardiomyocytes

FH

Zhuqing (Verified Customer) (11-26-2020)

In mouse cells, it can detect mature frataxin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 mouse myoblast

FH

Daniel (Verified Customer) (08-20-2019)

Works fine for WB in mouse brain and heart tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain