FXR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FXR1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (1)

Kat-Nr. : 13194-1-AP

Synonyme

FXR1, hFXR1p

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Galerie der Validierungsdaten

• 

  WB analysis using 13194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13194-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13194-1-AP (FXR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 13194-1-AP

  IP Result of anti-FXR1 (IP:13194-1-AP, 3ug; Detection:13194-1-AP 1:800) with mouse skeletal muscle tissue lysate 8000ug.

• 

  IHC staining of human heart using 13194-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13194-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13194-1-AP (FXR1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13194-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 13194-1-AP

  Immunofluorescent analysis of HepG2 cells using 13194-1-AP (FXR1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausherzgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: mit Ethacrynsäure behandelte HepG2-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• RIP: See 2 publications below

Produktinformation

13194-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA FXR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FXR1 fusion protein Ag3873
• Vollständiger Name: fragile X mental retardation, autosomal homolog 1
• Berechnetes Molekulargewicht: 621 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC028983
• Gene symbol: FXR1
• Gene ID (NCBI): 8087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.

Publikationen

Species	Application	Title
human	IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
mouse	IF	Neuron; Fmr1 KO and fenobam treatment differentially impact distinct synapse populations of mouse neocortex.; Authors - Gordon X Wang
mouse	IF	Nat Commun; Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle; Authors - Lance T. Denes
		Elife; Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses.; Authors - Gordon X Wang
human	RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez
mouse	WB	J Neurosci; Dysregulated RNA-Induced Silencing Complex (RISC) Assembly within CNS Corresponds with Abnormal miRNA Expression during Autoimmune Demyelination.; Authors - Przemysław Lewkowicz

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chao (Verified Customer) (01-30-2020)

Western blot show strong band in the basal state.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart