FXYD1 Monoklonaler Antikörper

FXYD1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C10G9

Kat-Nr. : 67150-1-PBS

Synonyme

FXYD1, Phospholemman, PLM

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  WB analysis of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 67150-1-Ig (same clone as 67150-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 67150-1-Ig (same clone as 67150-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67150-1-Ig (FXYD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67150-1-Ig (FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 67150-1-Ig (same clone as 67150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using FXYD1 antibody (67150-1-Ig, Clone: 2C10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67150-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67150-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA FXYD1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FXYD1 fusion protein Ag25307
• Vollständiger Name: FXYD domain containing ion transport regulator 1
• Berechnetes Molekulargewicht: 92 aa, 10 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC032800
• Gene symbol: FXYD1
• Gene ID (NCBI): 5348
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)