FXYD3 Polyklonaler Antikörper

FXYD3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15853-1-AP

Synonyme

Phospholemman like, MAT8, MAT 8, Mammary tumor 8 kDa protein, FXYD domain-containing ion transport regulator 3

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  WB analysis of COLO 320 using 15853-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15853-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 15853-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 15853-1-AP

  IP result of anti-FXYD3 (IP:15853-1-AP, 4ug; Detection:15853-1-AP 1:500) with COLO 320 cells lysate 2400ug.

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  IHC staining of human breast cancer using 15853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 15853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A375-Zellen, SGC-7901-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15853-1-AP bindet in WB, IHC, IP, ELISA FXYD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FXYD3 fusion protein Ag8672
• Vollständiger Name: FXYD domain containing ion transport regulator 3
• Berechnetes Molekulargewicht: 87 aa, 9 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: BC005238
• Gene symbol: FXYD3
• Gene ID (NCBI): 5349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.