Dysadherin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Dysadherin Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12166-1-AP

Synonyme

dysad, Dysadherin, FXYD5, HSPC113, IWU 1, IWU1, KCT1, OIT2, PRO6241, RIC, UNQ2561/PRO6241

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 12166-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12166-1-AP (Dysadherin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12166-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12166-1-AP (Dysadherin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12166-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12166-1-AP (Dysadherin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12166-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12166-1-AP (Dysadherin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 12166-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12166-1-AP (Dysadherin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 12166-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12166-1-AP (Dysadherin antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

12166-1-AP bindet in WB, IHC, ELISA Dysadherin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dysadherin fusion protein Ag2808
• Vollständiger Name: FXYD domain containing ion transport regulator 5
• Berechnetes Molekulargewicht: 178 aa, 19 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC009642
• Gene symbol: FXYD5
• Gene ID (NCBI): 53827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dysadherin, also known as FXYD5 (FXYD domain containing ion transport regulator 5), is a cancer-associated cell membrane glycoprotein which belongs to the FXYD family. The FXYD family, which contains seven members, are tissue specific regulators of the Na,K-ATPase. Dysadherin is involved in down-regulation of E-cadherin, which plays important roles in tumor development and metastasis. It is present in spleen, lung, skeletal muscle, and testis. Increased expression of dysadherin has been associated with increased cell motility and metastatic potential.

Publikationen

Species	Application	Title
		Int J Oncol; A FXYD5/TGF‑β/SMAD positive feedback loop drives epithelial‑to‑mesenchymal transition and promotes tumor growth and metastasis in ovarian cancer.; Authors - Yang Bai
human	WB,IHC	Cell Transplant; Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer.; Authors - Mi Tian
rat	WB	Bioengineered; FXYD domain containing ion transport regulator 5 (FXYD5) silencing promotes cell viability and alleviates inflammatory response in cerulein-induced AR42J cells by blocking JAK2/STAT3 signaling pathway.; Authors - Licheng Ding ; KD Validated
human	WB	Biochimie; Generation of the novel anti-FXYD5 monoclonal antibody and its application to the diagnosis of pancreatic and lung cancer; Authors - Takamasa Hotta ; KO Validated
human,mouse	WB	Mol Biotechnol; miR-1180 Targets FXYD5 to Regulate Pancreatic Cancer Cells Migration and Invasion; Authors - Hongmin Xie

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FXYD6 Polyclonal antibody

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FXYD4 Polyclonal antibody

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