FYN Polyklonaler Antikörper

FYN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17237-1-AP

Synonyme

Proto-oncogene c-Fyn, Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn

---

• 

  WB analysis using 17237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17237-1-AP (FYN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat testis using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17237-1-AP (FYN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17237-1-AP bindet in WB, IHC, ELISA FYN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FYN fusion protein Ag11080
• Vollständiger Name: FYN oncogene related to SRC, FGR, YES
• Berechnetes Molekulargewicht: 537aa,61 kDa; 482aa,55 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC032496
• Gene symbol: FYN
• Gene ID (NCBI): 2534
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FYN, also named p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family, and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing a greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.