Frizzled 5 Polyklonaler Antikörper

Frizzled 5 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (2)

Kat-Nr. : 21519-1-AP

Synonyme

C2orf31, Frizzled 5, Fz 5, FZD5, FzE5, HFZ5

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 21519-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21519-1-AP (FZD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21519-1-AP (Frizzled 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21519-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21519-1-AP (FZD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21519-1-AP (Frizzled 5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 21519-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21519-1-AP (FZD5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21519-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21519-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Frizzled 5 antibody (21519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, Jurkat-Zellen, Maus-Dünndarmgewebe, Maus-Kolongewebe, PC-12-Zellen, PC-3-Zellen, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

21519-1-AP bindet in WB, IHC, IF, ELISA Frizzled 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Frizzled 5 fusion protein Ag16082
• Vollständiger Name: frizzled homolog 5 (Drosophila)
• Berechnetes Molekulargewicht: 585 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC114346
• Gene symbol: FZD5
• Gene ID (NCBI): 7855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fzd5, is the receptor for the Wnt5A ligand. Fzd5 modulates cell fate and cell behavior during vertebrate development. It regulates hematopoiesis by the antagonism of the canonical Wnt pathway, resulting in a pool of quiescent hematopoietic stem cells, and regulates hematopoietic stem cell function in a paracrine fashion through several distinct mechanisms including modulating canonical Wnt signaling. It acts through noncanonical Wnt signaling to promote angiogenesis. It is also involved in control cell orientation, polarity, and directional movement in response to positional cues from chemokine gradients.

Publikationen

Species	Application	Title
human	WB	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu ; KD Validated
human	WB	Theranostics; Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.; Authors - Wenjia Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susanne (Verified Customer) (04-17-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Fibroblasts

Frizzled 5 Antibody Immunofluorescence validation (1:100 dilution) in Fibroblasts (Cat no:21519-1-AP)

FH

Louisiane (Verified Customer) (08-11-2022)

PFA fixed tissues (small intestine sections) were incubated over the weekend at 4C with primary antibody and overnight at 4C with secondary antibody. No signal detected in the small intestine.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 100