Frizzled 8 Polyklonaler Antikörper

Frizzled 8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 55093-1-AP

Synonyme

Frizzled 8, FZ 8, FZD8, hFZ8

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  WB analysis of HeLa using 55093-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse embryo using 55093-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells/tissue using 55093-1-AP

  WB result of 55093-1-AP (Frizzled 8 antibody).

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  WB analysis of mouse heart using 55093-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 55093-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 55093-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 55093-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausembryogewebe, Mausherzgewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

55093-1-AP bindet in WB, IHC, ELISA Frizzled 8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: frizzled homolog 8 (Drosophila)
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 70-73 kDa
• GenBank-Zugangsnummer: NM_031866
• Gene symbol: Frizzled 8
• Gene ID (NCBI): 8325
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
Human		Oncol Rep; HMQ‑T‑F2 suppresses migration of the human cervical cancer HeLa cells by reversing EMT via the PI3K/Akt signaling pathway.; Authors - Bingling Dai
human	WB	Theranostics; Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.; Authors - Wenjia Liu