Frizzled 9 Polyklonaler Antikörper

Frizzled 9 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13865-1-AP

Synonyme

CD349, Frizzled 9, Fz 9, FZD3, FZD9, FzE6, hFz9

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Galerie der Validierungsdaten

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  WB analysis using 13865-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13865-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13865-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13865-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13865-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 13865-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 13865-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13865-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13865-1-AP (Frizzled 9 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13865-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13865-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13865-1-AP (Frizzled 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HUVEC-Zellen, Maus-Augengewebe, Maushirngewebe, Maushodengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13865-1-AP bindet in WB, IHC,ELISA Frizzled 9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Frizzled 9 fusion protein Ag4847
• Vollständiger Name: frizzled homolog 9 (Drosophila)
• Berechnetes Molekulargewicht: 591 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC026333
• Gene symbol: FZD9
• Gene ID (NCBI): 8326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Oncol; YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.; Authors - Yang Bai
human	WB	Theranostics; Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.; Authors - Wenjia Liu