Factor IX/PTC Rekombinanter Antikörper

Factor IX/PTC Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241742G4

Kat-Nr. : 84533-4-PBS

Synonyme

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

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  WB analysis of human plasma using 84533-4-RR (same clone as 84533-4-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84533-4-RR (Coagulation factor IX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

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  IHC staining of Human Hepatocellular cancer using 84533-4-RR (same clone as 84533-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84533-4-RR (Factor IX/PTC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84533-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84533-4-PBS bindet in WB, IHC, Indirect ELISA Factor IX/PTC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Factor IX/PTC fusion protein Eg1045
• Vollständiger Name: coagulation factor IX
• Berechnetes Molekulargewicht: 52kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: NM_000133.4
• Gene symbol: F9
• Gene ID (NCBI): 2158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond. Native Factor IX MW is around 72kDa(PMID:827443; 12496253), and it can be cleaved into different fragments.