Factor XII Monoklonaler Antikörper

Factor XII Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E2F11

Kat-Nr. : 66089-1-PBS

Synonyme

F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1

---

• 

  WB analysis of K-562 using 66089-1-Ig (same clone as 66089-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using F12 antibody 66089-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66089-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Factor XII und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Factor XII fusion protein Ag3401
• Vollständiger Name: coagulation factor XII (Hageman factor)
• Berechnetes Molekulargewicht: 615 aa, 68 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC012390
• Gene symbol: Factor XII
• Gene ID (NCBI): 2161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.