Factor XIIIa Monoklonaler Antikörper

Factor XIIIa Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D2B2

Kat-Nr. : 66325-1-PBS

Synonyme

F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa

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  WB analysis using 66325-1-Ig (same clone as 66325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 66325-1-Ig (same clone as 66325-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit large intestine using 66325-1-Ig (same clone as 66325-1-PBS)

  rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66325-1-Ig (same clone as 66325-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 66325-1-Ig (same clone as 66325-1-PBS)

  rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66325-1-Ig (same clone as 66325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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  FC experiment of U-937 using 66325-1-Ig (same clone as 66325-1-PBS)

  1x10^6 U-937 cells were intracellularly stained with 0.25 ug Factor XIIIa Monoclonal antibody (66325-1-Ig, Clone:2D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66325-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66325-1-PBS bindet in WB, IHC, Indirect ELISA Factor XIIIa und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Factor XIIIa fusion protein Ag11105
• Vollständiger Name: coagulation factor XIII, A1 polypeptide
• Berechnetes Molekulargewicht: 732 aa, 83 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC027963
• Gene symbol: Factor XIIIa
• Gene ID (NCBI): 2162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.