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Fas/CD95 Polyklonaler Antikörper

Fas/CD95 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30601-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (30601-1-AP)

    WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Fas/CD95 Polyclonal antibody (30601-1-AP)

    WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using Fas/CD95 Polyclonal antibody (30601-1-AP)

    IF Staining of Raji using 30601-1-AP (same clone as 30601-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30601-1-PBS bindet in WB, IF/ICC, Indirect ELISA Fas/CD95 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Fas/CD95 fusion protein Ag30426
Vollständiger Name Fas (TNF receptor superfamily member 6)
Berechnetes Molekulargewicht 37 kDa
Beobachtetes Molekulargewicht 30-40 kDa
GenBank-ZugangsnummerNM_007987
Gene symbol Fas/CD95
Gene ID (NCBI) 14102
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.