Fas/CD95 Rekombinanter Antikörper

Fas/CD95 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240340E11

Kat-Nr. : 98034-1-PBS

Synonyme

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

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  FC experiment of human PBMCs using 98034-1-RR (same clone as 98034-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed. This data was developed using the same antibody clone with 98034-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98034-1-RR (same clone as 98034-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR, Clone: 240340E11) (red), or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98034-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98034-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98034-1-RR against Human Fas/CD95 were performed. The affinity constant is 1.97 nM.

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Geprüfte Anwendungen

Produktinformation

98034-1-PBS bindet in FC Fas/CD95 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: Fas (TNF receptor superfamily, member 6)
• Berechnetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_000043.6
• Gene symbol: Fas/CD95
• Gene ID (NCBI): 355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.