Fas/CD95 Rekombinanter Antikörper

Fas/CD95 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

240340E11

Kat-Nr. : Biotin-FcA98034

Synonyme

FAS, ALPS1A, APO-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS

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  FC experiment of human PBMCs using Biotin-FcA98034

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human Fas/CD95 Rabbit RecAb (Biotin-FcA98034, Clone: 240340E11) and PE Streptavidin (Cat.No. PF00021), or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) and PE Streptavidin(Cat.No. PF00021). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-FcA98034

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human Fas/CD95 Rabbit RecAb (Biotin-FcA98034, Clone: 240340E11) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-FcA98034 bindet in FC Fas/CD95 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fas/CD95 fusion protein Eg0877
• Vollständiger Name: Fas (TNF receptor superfamily, member 6)
• Berechnetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_000043.6
• Gene symbol: Fas/CD95
• Gene ID (NCBI): 355
• Konjugation: Biotin
• Excitation/Emission maxima wavelengths: -
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.