Fas/CD95 Rekombinanter Antikörper

Fas/CD95 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241229C11

Kat-Nr. : CL647-98135

Synonyme

CD95, Fas, 241229C11, APO 1, APT1

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  FC experiment of mouse thymocytes using CL647-98135

  1x10^6 mouse thymocytes were surface stained with CoraLite® Plus 405 Anti-Mouse CD8a, and 0.25 ug CoraLite® Plus 647 Anti-Mouse Fas/CD95 Rabbit RecAb (CL647-98135, Clone: 241229C11) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse thymocytes using CL647-98135

  1x10^6 mouse thymocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Fas/CD95 Rabbit RecAb (CL647-98135, Clone: 241229C11) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Thymozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98135 bindet in FC Fas/CD95 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fas/CD95 fusion protein Eg1376
• Vollständiger Name: Fas (TNF receptor superfamily member 6)
• Berechnetes Molekulargewicht: 37kDa
• GenBank-Zugangsnummer: NM_007987.2
• Gene symbol: Fas/CD95
• Gene ID (NCBI): 14102
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.