Ferritin heavy chain Polyklonaler Antikörper

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• KD/KO Validated

Ferritin heavy chain Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11682-1-PBS

Synonyme

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

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  WB analysis using 11682-1-AP (same clone as 11682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  WB result of Ferritin heavy chain antibody (11682-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  WB analysis using 11682-1-AP (same clone as 11682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  IP result of anti-Ferritin heavy chain (IP:11682-1-AP, 4ug; Detection:11682-1-AP 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11682-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Ferritin heavy chain und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ferritin heavy chain fusion protein Ag2290
• Vollständiger Name: ferritin, heavy polypeptide 1
• Berechnetes Molekulargewicht: 183 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000857
• Gene symbol: FTH1
• Gene ID (NCBI): 2495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.