Ferritin heavy chain Rekombinanter Antikörper

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• KD/KO Validated

Ferritin heavy chain Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

230312F3

Kat-Nr. : 83428-1-RR

Synonyme

Ferritin, FTH1, 230312F3, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1

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  WB analysis of HeLa using 83428-1-RR

  WB result of Ferritin heavy chain antibody (83428-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells.

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  WB analysis using 83428-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83428-1-RR (Ferritin heavy chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83428-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (83428-1-RR, Clone: 230312F3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83428-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83428-1-RR against Human FTH1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Lebergewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

83428-1-RR bindet in WB, IF/ICC, ELISA Ferritin heavy chain und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ferritin heavy chain fusion protein Ag2290
• Vollständiger Name: ferritin, heavy polypeptide 1
• Berechnetes Molekulargewicht: 183 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000857
• Gene symbol: FTH1
• Gene ID (NCBI): 2495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

Publikationen

Species	Application	Title
rat	WB	Eur J Pharm Sci; Pharmacodynamic Insights into Maresin 1: Enhancing Flap Viability via the Keap1/Nrf2 Axis to Control ROS-Driven Apoptosis and Ferroptosis; Authors - Pin Fang
human	WB	Cancers (Basel); Mitochondrial Iron Metabolism as a Potential Key Mediator of PD-L1 Thermal Regulation; Authors - Gizzy Keeler
	WB	Int Immunopharmacol; Urolithin A improves myocardial ischemia-reperfusion injury by attenuating oxidative stress and ferroptosis through Nrf2 pathway; Authors - Zhe Su
human	WB	Cell Death Dis; ZIP8 modulates ferroptosis to drive esophageal carcinoma progression; Authors - Zhaojie Yang
mouse	WB	iScience; ROS-responsive nanoparticles with selenomethionine for ferroptosis modulation in abdominal aortic aneurysm; Authors - Haipeng He
	WB	Viruses; Infectious Spleen and Kidney Necrosis Virus Triggers Ferroptosis in CPB Cells to Enhance Virus Replication; Authors - Qiushuang Zhang