Ferritin light chain Monoklonaler Antikörper

Ferritin light chain Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9F5

Kat-Nr. : 68068-1-PBS

Synonyme

Ferritin L subunit, Ferritin light chain, ferritin, light polypeptide, FTL

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  WB analysis of A549 using 68068-1-Ig (same clone as 68068-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68068-1-Ig (same clone as 68068-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68068-1-Ig (same clone as 68068-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68068-1-Ig (Ferritin light chain antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68068-1-Ig (same clone as 68068-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FTL antibody (68068-1-Ig, Clone: 1F9F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68068-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68068-1-PBS bindet in WB, IHC, IF/ICC, ELISA Ferritin light chain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ferritin light chain fusion protein Ag20637
• Vollständiger Name: ferritin, light polypeptide
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC008439
• Gene symbol: Ferritin light chain
• Gene ID (NCBI): 2512
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.