Fetuin-A/AHSG Monoklonaler Antikörper

Fetuin-A/AHSG Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6B9

Kat-Nr. : 66094-1-PBS

Synonyme

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

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  WB analysis of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66094-1-Ig (same clone as 66094-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66094-1-Ig (same clone as 66094-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  IP result of anti-Fetuin-A (IP:66094-1-Ig, 3ug; Detection:66094-1-Ig 1:3000) with HepG2 cells lysate 6000ug. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Fetuin-A antibody (66094-1-Ig, Clone: 1F6B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig, Clone:1F6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66094-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Fetuin-A/AHSG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fetuin-A/AHSG fusion protein Ag10073
• Vollständiger Name: alpha-2-HS-glycoprotein
• Berechnetes Molekulargewicht: 367 aa, 39 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC048198
• Gene symbol: Fetuin-A
• Gene ID (NCBI): 197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.